Methods For In-House Preparation of PRP Serum 

PRP or Platelet Rich Plasma therapy is a non-invasive and non-surgical treatment that has gained enough popularity in the medicinal and cosmetic practice. As a result of this rising demand, medical professionals are looking for ways to offer high-quality PRP treatments at reasonable costs to their patients. Clinicians are looking for ways to design affordable in-house PRP preparation since commercially available PRP kits vary in capacity for sufficiently concentrated viable platelets.

Best commercial PRP tubes at reasonable rates

Dr. Akhmerov’s Plasmolifting Technologies offers the best PRP tubes Italy at reasonable rates for the production of platelet-rich plasma. This is an innovative healthcare company with PRP tubes as their core product that is already been used successfully by thousands of physicians worldwide for various therapeutic purposes. The PRP tubes that the company recommends ensure their method’s security since they meet all the technical specifications set by their expert developers and all the regulatory needs concerning medical devices.

Different approaches for making in-house PRP serum

Studies reveal that there are two distinct approaches for preparing low-cost PRP preparation techniques for producing a standardized platelet concentration.

Techniques for standardizing PRP

  • 55 ml of blood was withdrawn from 18 healthy volunteers in 6 different vacuum tubes.
  • Entire blood was extracted through centrifugation and 1 mm of blood was taken for platelet analysis.
  • The PRP serum was prepared with two rounds of centrifugation.
  • Red blood cells sink at the bottom of the vacuum tubes that contains the platelets.
  • The leftover is blood plasma.
  • Dual centrifugation is carried out in another sterile vacuum tube that further boosts platelet concentration. 
  • After the second spin, 1.5 ml of concentrated PRP is left behind.

Researchers discovered that all samples had a better platelet concentration. This proved that the in-house method for creating platelet-rich serum was successful. 

How far are PRP injections safe?

Plenty of research shows that injections of in-house PRP serum are safe and promote the healing of wounds and damaged tissues. However, determining the exact therapeutic doses will need more clinical trials with proper serum and growth factor evaluations. For instance, the serum obtained from the lower force secondary centrifugation might not work for treating osteoarthritis.

Disposable syringes for preparing PRP

  • The second technique made PRP using standard 5 ml syringes for which the 4ml of blood sample was divided into 16 syringes. 
  • The blood was centrifuged for 3 minutes at 3000rpm. 
  • Secondary centrifugation was performed for 15 minutes at 4000 rpm.
  • The leftover was discarded leaving behind .65 ml of PRP in each syringe.

While the method utilizes the available material but working with such small amounts of PRP serum may be challenging as it does not deliver an adequate amount of platelets for therapeutic purposes.

Developing an in-house PRP serum may be appealing to clinicians which might increase the availability of homemade PRP serum. However, studies are still going on to verify the safety of non-medical equipment. This is why clinicians still prefer licensed and reputed PRP kit suppliers for extracting platelet-rich plasma from autologous blood using PRP tubes so that patients can get the most from their PRP treatment.